LOCUS       pLV-[hsa-miR-376a-2]        9134 bp    DNA     circular      3-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7055..7915
                     /label=Amp(R)
     promoter        8116..8656
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5660..5894
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6469..6924)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5966..6096
                     /label=SV40 pA
     misc_feature    6097..6310
                     /label=SV40 ori
     misc_feature    4981..5577
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3599
                     /label=intron
     CDS             3646..4971
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3389
                     /label=hsa-mir-376a-2 precursor
BASE COUNT     2158 a      2357 c      2484 g      2135 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc attttctcca 
     3121 tgaggccgaa tgctctcctc tgtgctatgt tacttttgtg tttgatggat tgtacttagg 
     3181 ttcgtgcttt ccgggatgaa accttctttg gtatttaaaa ggtagatttt ccttctatgg 
     3241 ttacgtgttt gatggttaat catagaggaa aatccacgtt ttcagtatca aatgctgcct 
     3301 tgggatcatg gctggactga agccaccatc agggacgtga ggtggggctg agccctccag 
     3361 ggtaagtgga aagatggtgg gccgcagaag ctagctcgag cttttggagt acgtcgtctt 
     3421 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg 
     3481 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg 
     3541 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg 
     3601 tgtcgtgaaa actacccctc tagagtcgag ctaccggtcg ccaccatggt gagcaagggc 
     3661 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 
     3721 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 
     3781 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 
     3841 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 
     3901 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 
     3961 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac 
     4021 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 
     4081 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 
     4141 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 
     4201 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 
     4261 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 
     4321 cactccaccg gcggcatgga cgagctgtac aaggacccac cggtcgccac catgaccgag 
     4381 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 
     4441 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 
     4501 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 
     4561 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 
     4621 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 
     4681 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 
     4741 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 
     4801 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 
     4861 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 
     4921 tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc ccggtgcctg agcggccgcg 
     4981 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 
     5041 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 
     5101 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 
     5161 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 
     5221 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 
     5281 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 
     5341 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 
     5401 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 
     5461 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 
     5521 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 
     5581 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 
     5641 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct 
     5701 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 
     5761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 
     5821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 
     5881 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 
     5941 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 
     6001 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     6061 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 
     6121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     6181 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 
     6241 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt 
     6301 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 
     6361 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 
     6421 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta 
     6481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     6541 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     6601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 
     6661 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 
     6721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     6781 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 
     6841 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 
     6901 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc 
     6961 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 
     7021 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 
     7081 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 
     7141 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 
     7201 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 
     7261 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 
     7321 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 
     7381 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 
     7441 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 
     7501 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 
     7561 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 
     7621 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 
     7681 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 
     7741 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 
     7801 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 
     7861 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 
     7921 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     7981 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     8041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     8101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     8161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     8221 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     8281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     8341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     8401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     8461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     8521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     8581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     8641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     8701 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 
     8761 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 
     8821 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct 
     8881 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 
     8941 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 
     9001 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 
     9061 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca 
     9121 aaagctggag ctgc 
//
